reference strain b cereus atcc 14579  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Organic vs. Conventional Milk: Uncovering the Link to Antibiotic Resistance in Bacillus cereus sensu lato

doi: 10.3390/ijms252413528

Figure Lengend Snippet: Variability in the relative expression levels of three enterotoxin genes of Bacillus cereus sensu lato among strains isolated from conventional milk (yellow boxes) and organic milk (blue boxes). Values are referenced against the expression levels of nheA , hblA , and cytK genes in the reference strain B. cereus ATCC 14579 (value of 1). Circles represent mean values, diamonds indicate medians, and boxes illustrate the range of values.

Article Snippet: In the qPCR analyses, we found that the toxigenic potential of the studied bacteria was comparable to the reference B. cereus ATCC 14579 strain, although certain isolates could biosynthesize toxins at much higher levels than the reference one.

Techniques: Expressing, Isolation

Journal: International Journal of Molecular Sciences

Article Title: Organic vs. Conventional Milk: Uncovering the Link to Antibiotic Resistance in Bacillus cereus sensu lato

doi: 10.3390/ijms252413528

Figure Lengend Snippet: The percentage of unique alleles for the genes glp , gmk , ilv , pta , pur , pyc , and tpi among 90 identified sequence types of Bacillus cereus sensu lato. The values above the bars indicate the number of different alleles for each gene.

Article Snippet: In the qPCR analyses, we found that the toxigenic potential of the studied bacteria was comparable to the reference B. cereus ATCC 14579 strain, although certain isolates could biosynthesize toxins at much higher levels than the reference one.

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Organic vs. Conventional Milk: Uncovering the Link to Antibiotic Resistance in Bacillus cereus sensu lato

doi: 10.3390/ijms252413528

Figure Lengend Snippet: The population structure of Bacillus cereus sensu lato isolated from conventional and organic milk, determined using goeBURST analysis. Green circles represent sequence types (STs) corresponding to reference strains, blue circles represent STs of strains from organic milk, red circles represent STs of strains from conventional milk, and yellow circles represent STs represented by strains from both types of milk. Stars indicate central isolates within clonal complexes that have central isolates. Bold and underlined numbers represent STs corresponding to strains resistant to at least three classes of antibiotics, while underlined numbers correspond to STs of strains resistant to two classes of antibiotics.

Article Snippet: In the qPCR analyses, we found that the toxigenic potential of the studied bacteria was comparable to the reference B. cereus ATCC 14579 strain, although certain isolates could biosynthesize toxins at much higher levels than the reference one.

Techniques: Isolation, Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Organic vs. Conventional Milk: Uncovering the Link to Antibiotic Resistance in Bacillus cereus sensu lato

doi: 10.3390/ijms252413528

Figure Lengend Snippet: Phylogenetic relationship of Bacillus cereus s.l. reconstructed using MEGA 7 software (Maximum Likelihood method, GTR G+I model) based on concatenated sequences of seven housekeeping genes, following the MLST scheme in the PubMLST service .

Article Snippet: In the qPCR analyses, we found that the toxigenic potential of the studied bacteria was comparable to the reference B. cereus ATCC 14579 strain, although certain isolates could biosynthesize toxins at much higher levels than the reference one.

Techniques: Software